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Julian ng drosophila. Facebook gives people the power to share Director in the Healthcare & Life Sciences practice at West Monroe Partners. Jefferis, Sebastian Cachero, Luke D. A highly related Mig-2-like (Mtl) gene, the orthologue of Caenorhabditis elegans mig-2 (ref. Dec 19, 2016 · Request PDF | Second Generation Drosophila Chemical Tags: Sensitivity, Versatility and Speed | Labeling and visualizing cells and sub-cellular structures within thick tissues, whole organs and May 30, 2018 · JUN 19, 2018 Dissection and Fixation of Drosophila CNS Geoffrey W. […] Aug 15, 2010 · With the goal of discovering genes that regulate the neuronal morphogenesis in the postembryonic nervous system, we conducted a pilot misexpression screen in adult Drosophila neurons. Passionate about multidisciplinary interactions that can drive healthcare and life science innovations. Johnston, Ben Sutcliffe, Julian Ng, Gregory S. Feb 14, 2017 · We now present a second generation of UAS- and LexA-responsive CLIPf, SNAPf, and Halo chemical labeling reagents for flies. Auer Mathias Pasche Richard Benton Gregory S. Meta Aug 15, 2018 · To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. Dec 19, 2016 · December 19, 2016 5 Ben Sutcliffe1,* , Julian Ng*,†, Thomas O. Unknown affiliation - Cited by 214 - research - immuno-oncology - biotechnology - science communication Ben Sutcliffe Julian Ng Thomas O. Andrew Rallis, Coralie Moore, and Julian Ng⁎ MRC Centre for Developmental Neurobiology, New Hunt's House, Guy's Campus, King's College, London SE1 1UL, UK. Mar 5, 2021 · It’s all about precision for Grand Venture Technology CEO Julian Ng, whether it’s running a marathon, cooking a meal or using technology to improve the lives of others. We found that misexpression of the transcription factor hindsight (hnt) in atonal neurons of the adult fly, affects the axonal guidance and dendritic arborization. Keywords: mcfo ihc of drosophila cn, chemical tagging of neuron, drosophila cn, drosophila brain, improved drosophila melanogaster transgene, drosophila melanogaster transgene, chemical fluorophore ligand, rapid labeling of specific cell, new fluorophore ligand, labeling technique with standard immunohistochemistry, chemical tagging, neuron Apr 17, 2025 · The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, MRC Centre for Developmental Neurobiology, King's College (8/05). Dec 13, 2016 · Article Open access Published: 13 December 2016 Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG Julian Ng, Alyssa Browning May 29, 2007 · Perspective Published: 29 May 2007 Using FlyAtlas to identify better Drosophila melanogaster models of human disease Venkateswara R Chintapalli, Jing Wang & Julian A T Dow Nature Genetics 39, 715 The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, University of Cambridge. Feb 1, 2013 · Molecular chaperones protect against JNK- and Nmnat-regulated axon degeneration in Drosophila Andrew Rallis, Bingwei Lu, Julian Ng Author and article information Mar 28, 2002 · In particular, Rac has been implicated in axon growth and guidance2,3,4,5,6,7,8. et al. E. I found that components of the Wnt/Planar Cell Polarity (PCP) pathway control MB axon branching Julian Ng, Alyssa Browning, Lorenz Lechner, Masako Terada, Gillian Howard, Gregory S. Find your dream home or connect with Julian Ng for personalized real estate advice and local market insights in Banning, CA. Goldstein, Yan Sun, Georg Dietzl, Barry J. We found that Acknowledgments We thank Robert Ray, Mike O'Connor, Julian Ng, Dominique Ferrandon, the Vienna Drosophila RNAi Center, and the Bloomington Drosophila Stock Center for providing flies. Harms, Julia Tzu, A. View the profiles of professionals named "Julian Ng" on LinkedIn. Johnston 1 ,Ben Sutcliffe 2 ,Julian Ng 2 , Gregory S. , 1999; Ng et al. TagRFP. COM Diogo Jota: 1996 Key applications for chemical labeling in Drosophila neurobiology. To understand how this develops, I analyzed the stereotyped pattern of Drosophila mushroom body (MB) neurons, which have single axons branches that extend dorsally and medially. We describe the major developmental events that build these organs during embryogenesis, remodel them during metamorphosis, and repair them following injury. We found Mar 28, 2002 · Here we analyse the loss-of-function phenotypes of three Rac GTPases in Drosophila mushroom body neurons. View Julian Ng results in California including current home address, phone number, relatives & property records with Whitepages. These topic labels come from the works of this person. View the profiles of people named Julian Ng. Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG Preprint Full-text available Aug 2016 Julian Ng Alyssa Browning Lorenz Lechner Venkateswara R Chintapalli, Jing Wang & Julian A T Dow FlyAtlas, a new online resource, provides the most comprehensive view yet of expression in multiple tissues of Drosophila melanogaster. frizzled Here we analyse the loss-of-function phenotypes of three Rac GTPases in Drosophila mushroom body neurons. ensemble @flueremusic @studioaestus @meritmusic" Julian Ng is the Co-founder and Chief Executive Officer of Grand Venture Technology Limited since March 2014, recognized as the EY Entrepreneur of the Year 2022 in Advanced Manufacturing. Jefferis 2 ,Sebastian Cachero 2 ,Luke D The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. Jonathan B. Auer‡, Mathias Pasche**, Richard Benton In addition to functioning as an intestinal valve, the pylorus is also an important zone of interaction between the Drosophila host environment and its microbiota, both symbiotic and pathogenic. In the complete absence of Drosophila JNK (Basket), MB axons fail to stabilize, leading to their subsequent degeneration. org Real Estate Agent profile of Julian Ng on Realty. Meissner 1 ,Jonathan B. View Julian Ng’s profile on LinkedIn, a professional community of 1 billion members. Drosophila JNK loss results in age-dependent axon degeneration. Dive into the research topics where Julian Ng is active. Drosophila constructs and transgenic flies Drosophila transformation plasmids from Table 1 were made by Gibson assembly (Gibson et al. Download scientific diagram | Key applications for chemical labeling in Drosophila neurobiology (a) Map of the brp-SNAP knock-in. Auer,Mathias Pasche,Richard Benton,Gregory S. Meiss ner , Jonathan B. I’ve written various musicals and plays over the years, and continue to write either in collaboration with others or alone. Second Generation Drosophila Chemical Tags: Sensitivity, Versatility and Speed Preprint Dec 2016 Ben Sutcliffe Julian Ng Thomas Oliver Auer [] Sebastian Cachero Abstract The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. 2017. Here we show that the actin depolymerization factor cofilin is essential for axon growth in Drosophila neurons. Description: Step up targeted patrols in AAPI communities as well as businesses and gathering spots Service Area: SF, specific neighborhoods Contact: Julian Ng Phone: 415-315-2400 Email: julian. D in immunology and cancer biology. Grimm 1 ,Rebecca M. Feb 16, 2017 · Second Generation Drosophila Chemical Tags:1 Sensitivity, V ersatility and Speed 2 3 F ebruary 10, 2017 4 Ben Sutcliffe 1,* , Julian Ng *,†, Thomas O. Feb 1, 2013 · Axon degeneration is observed at the early stages of many neurodegenerative conditions and this often leads to subsequent neuronal loss. 😁😁 19 19 Julian Ng Jul 3 YNWA, may the peace, strength and the Lord be with your family and you in heaven! LIVERPOOLFC. Oct 2, 2025 · The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, University of Cambridge. As a passionate entrepreneur, I thrive on transforming innovative ideas into impactful… · Experience: Grand Venture Technology Limited · Education: Ngee Ann Polytechnic · Location: Singapore · 500+ connections on LinkedIn. D, describes the new methods pioneered in his recent paper and the impact it will have on the new field of connectomics. Jefferis December 19, 2016 5 Ben Sutcliffe1,* , Julian Ng*,†, Thomas O. Auer‡, Mathias Pasche*, Richard Benton‡, Regulators of axon guidance pathways in the Drosophila brainNg, Julian (Primary Investigator) Project: Research Overview Julian Ng has built a robust career in finance and strategy, currently serving as Senior Manager in Finance & Strategy at Pilot. Oct 11, 2011 · Acknowledgments We thank Robert Ray, Mike O'Connor, Julian Ng, Dominique Ferrandon, the Vienna Drosophila RNAi Center, and the Bloomington Drosophila Stock Center for providing flies. Dickson, Liqun Luo Rac function and regulation during Drosophila development A Maritime and Office Professional Recruitment Specialist with many years of experience… · Education: Nanyang Technological University · Location: Singapore · 500+ connections on LinkedIn. Apr 1, 2017 · Drosophila constructs and transgenic flies Drosophila transformation plasmids from Table 1 were made by Gibson assembly (Gibson et al. org or SFPDCentralStation@sfgov. Auer‡, Mathias Pasche*, Richard Benton‡, The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. Jefferis, Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG, Scientific Reports, 6, 1, (2016). Figures S4 to S20 in File S1 show the primers and enzymes used to make each plasmid. . Ng J, Browning A, Lechner L, Terada M, Howard G, Jefferis GSXE Recently we developed an alternative approach using genetically encoded chemical tags CLIP, SNAP, Halo and TMP for tissue labeling; this resulted in >100 fold increase in labeling speed in both mice and Drosophila, at the expense of a considerable drop in absolute sensitivity when compared to optimized immunofluorescence Dec 13, 2016 · How to cite this article: Ng, J. The Drosophila genome has two Rac genes that share 92% amino acid sequence identity and have overlapping expression patterns2,9,10. YFP}16. Grimm, Rebecca M. Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG. We previously showed that inactivating the c-Jun N-terminal kinase (JNK) pathway leads to axon degeneration in Drosophila mushroom body (MB) neurons. Dec 13, 2016 · Authors Julian Ng 1 2 , Alyssa Browning 3 , Lorenz Lechner 3 , Masako Terada 3 , Gillian Howard 4 , Gregory S X E Jefferis 1 2 Abstract Branching morphology is a hallmark feature of axons and dendrites and is essential for neuronal connectivity. Grimm , Rebecca M. Auer,‡ Mathias Pasche,§Richard Benton Julian is a Registered Foreign Lawyer in Dentons’ Litigation & Dispute Resolution group based in Hong Kong. , 2002), and standard recombination techniques were used to generate mutant strains. We show that progressive loss of combined Rac1, Rac2 and Mtl activity leads first to defects in axon branching, then guidance, and finally growth. P {UAS-wit. P {UAS-Eb1. View Julian Ng (IHRP-SP)’s profile on LinkedIn, a professional community of 1 billion members. We found that Aug 22, 2016 · Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG Julian Ng, Alyssa Browning, Lorenz Lechner, Masako Terada, Gillian Howard, Gregory S. These multimerized tags, with translational enhancers, display up to 64-fold increase in sensitivity over first-generation reagents. Axon guidance in the Drosophila brain: Rho GTPases and cytoskeletal regulatory pathways. The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. Julian Ng, Timothy Nardine, Matthew B. , 2012). ai, ETH Zürich, HHMI Janelia Julian Ng Leiden University Jan Funke HHMI Janelia Research Campus Scott Waddell University of Oxford Markus William Pleijzier Laboratory of Molecular Biology, Cambridge Hatching-Box: Automated in situ monitoring of Drosophila melanogaster development in standard rearing vials Julian Bigge, Maite Ogueta, Luis Garcia, Benjamin Risse Here, focusing on discoveries in the past 10 years, we provide an overview of the anatomy and physiology of the Drosophila excretory system. X. ng@sfgov. To demonstrate this utility more clearly, we optimized Drosophila transgenics for applications that are at the heart of large-scale studies of neural circuits in the fly brain. We found 13 people named Julian Ng in California. 2018) reviewed recent progress on Drosophila intestinal microbiota. (A) Comparison between epitope-tagged synaptic markers (HA-tag, mCherry) and synaptically targeted chemical tags (SNAP-tag, CLIP Jan 13, 2012 · We are grateful to Akiko Miyoshi, Yusei Kawahara, Wang Honghang and the Gene Technology Center, Kumamoto University, for technical assistance; Drs Takashi Suzuki, Julian Ng, Linda Partridge, the Bloomington Drosophila Stock Center at Indiana University, the Drosophila Genetic Resource Center (DGRC), Kyoto, the Vienna Drosophila RNAi Center Acknowledgments We thank Robert Ray, Mike O'Connor, Julian Ng, Dominique Ferrandon, the Vienna Drosophila RNAi Center, and the Bloomington Drosophila Stock Center for providing flies. Jefferis Michael-John Dolan Department of Genetics, Trinity College Dublin, Ireland Richard Benton University of Lausanne Nils Eckstein Kaiko. 2009) (Figures S4 to S13 in File S1) or restriction enzyme cloning (Figures S14 to S20 in File S1) and deposited at Addgene. ” The Drosophila reagents that we have described here have immediate and widespread utility in cell and developmental biology and neurobiology. (A–C) Adult MB JNK (bsk147e) neuroblast clones exhibit axon degeneration with axon thinning and terminal fragmentation in β- (yellow arrows) and γ-axons (blue arrows). Feb 9, 2017 · New practical approach facilitates brain mapping from the level of the synapse up. Where possible, hoping to turn basic Dissection and Fixation of Drosophila CNS Geoffrey W. Recently, we developed an alternative approach using genetically encoded chemical tags CLIP, SNAP, Halo, and TMP for tissue labeling; this resulted in >100-fold increase in labeling speed in both mice and Drosophila, at the expense of a considerable drop in absolute sensitivity when compared to optimized immunofluorescence staining. P{UAS-Mkk4. Ph. Together they form a unique fingerprint. Drosophila strains for MARCM analysis have been previously described (Lee et al. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila mel- anogaster transgenes. We previously showed that inactivating the c-Jun N-terminal kinase (JNK) pathway leads to axon degeneration in Drosophila Aug 15, 2018 · Despite the utility of chemical tagging in tissue, the existing collection of commercial fluorophore ligands was not developed explicitly for fixed tissue labeling. X. Auer‡, Mathias Pasche*, Richard Benton‡, Here we show activity levels and timing mechanisms determine distinct aspects of Jun N-terminal kinase (JNK) pathway dependent axonal morphogenesis in Drosophila mushroom body (MB) neurons. Jefferis Sebastian Cachero Regulatory Affairs | Biotechnology | Biopharmaceutical Research · Research scientist with broad accomplishments and expertise in bioimaging, cell biology, cell signalling, molecular genetics and neuroscience. 774 Followers, 760 Following, 35 Posts - Julian Ng (@jujunotogame) on Instagram: "RCM ‘22 CCPA ‘24 @ikaros. Here we analyse the loss-of-function phenotypes of three Rac GTPases in Drosophila mushroom body neurons. Here, we evaluate chemical tagging to replace or complement IHC labeling of neurons in the Drosophila melanogaster central nervous system. hnt encodes a nuclear zinc-finger protein that Jun 9, 2022 · A dinner in February celebrated the promotions of top Chinese American police officers, Julian Ng (from left), Paul Yep, David Lazar and Daryl Fong. Halo2} expresses the E isoform of Eb1 protein fused at its C-terminus to tagRFP and Halo2 under UASt control. We found that tissue Dec 4, 2024 · Sutcliffe, Ben, Julian Ng, Thomas O Auer, Mathias Pasche, Richard Benton, Gregory S X E Jefferis, and Sebastian Cachero. Join Facebook to connect with Julian Ng and others you may know. Here, focusing on discoveries in the past 10 years, we provide an overview of the anatomy and physiology of the Drosophila excretory system. “Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed. “Julian provided invaluable guidance, offering insightful feedback to the Management that significantly contributed to the overall quality and efficiency of our operations. There are 100+ professionals named "Julian Ng", who use LinkedIn to exchange information, ideas, and opportunities. Cofilin function in axon growth is inhib … Dec 19, 2016 · December 19, 2016 5 Ben Sutcliffe1,* , Julian Ng*,†, Thomas O. Mar 28, 2002 · We used these loss-of-function mutations to assess the contribution of each Rac protein to a set of distinct cell-shape changes that occur during Drosophila development. 1 is a homozygous viable and fertile, second chromosome insertion. ” Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed Ben Sutcliffe,*,1 Julian Ng,*,† Thomas O. To understand … Feb 1, 2013 · Axon degeneration is observed at the early stages of many neurodegenerative conditions and this often leads to subsequent neuronal loss. Jefferis Scientific Reports（2016） 引用27浏览0WOSNATURE引用 27 0 Second-Generation Drosophila Chemical Tags: Sensitivity, Versatility, and Speed Ben Sutcliffe,Julian Ng,Thomas O. Leiden University - Cited by 1,949 - Molecular Biology - Cell Biology - Assay Technologies - Disease Models Genetically targeted 3D visualisation of Drosophila neurons under Electron Microscopy and X-Ray Microscopy using miniSOG. The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, University of Cambridge. The fusion protein localizes to the cytoplasm. Leader in… · Experience: Affordable Care · Education: Dartmouth College · Location: Chicago · 500 1 1 Julian Ng Jul 10 Hi Guys, I will be in Suntec till Sunday, if you want to know the latest information (Kang Tau) in Malaysia property come visit me. I found that components of the Wnt/Planar Cell Polarity (PCP) pathway control MB axon branching Leiden University - 引用次数：2,030 次 - Molecular Biology - Cell Biology - Assay Technologies - Disease Models Jul 24, 2025 · The following information accompanied stocks donated to the Bloomington Stock Center by Julian Ng, MRC Centre for Developmental Neurobiology, King's College (8/05). Lavis, Oz Malkesman 1 Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, USA; 2 Division of Neurobiology, Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom Branching morphology is a hallmark feature of axons and dendrites and is essential for neuronal connectivity. To understand how this develops, I analyzed the stereotyped pattern of Drosophila mushroom body (MB) neurons, which have single axons branches that extend dorsally and medially. Lily Lo Robert Chiang congratulated them. E. I found that components of the Wnt/Planar Cell Polarity (PCP) pathway control MB axon branching. Jefferis,Sebastian Large dimension, high-resolution imaging is important for neural circuit visualisation as neurons have both long- and short-range patterns: from axons and dendrites to the numerous synapses at Julian Ng, Alyssa Browning, Lorenz Lechner, Masako Terada, Gillian Howard and Gregory S. Lead Author Julian Ng, Ph. Julian completed his training contract with Dentons’ London office and is admitted to practice as a solicitor in England & Wales. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and tested these with recently improved Drosophila melanogaster transgenes. Flybase nomenclature is used for all Drosophila genes throughout the paper. com. Abstract Branching morphology is a hallmark feature of axons and dendrites and is essential for neuronal connectivity. (b) nc82 and brp-SNAP signals co-localize in a brp-SNAP/+ brain Dec 2, 2004 · Rho GTPases are essential regulators of cytoskeletal reorganization, but how they do so during neuronal morphogenesis in vivo is poorly understood. Dec 2, 2024 · Sutcliffe, Ben, Julian Ng, Thomas O Auer, Mathias Pasche, Richard Benton, Gregory S X E Jefferis, and Sebastian Cachero. A recent FlyBook chapter (Miguel-Aliaga et al. A further report suggests Drosophila Nmnat (dNmnat or nmnat) also controls axonal mitochondria levels and their availability is key to neuroprotection following acute injury (Fang et al. Jefferis , Sebastian Cachero Julian Ng,Alyssa Browning,Lorenz Lechner,Masako Terada,Gillian Howard,Gregory S. Venus} expresses wit protein with a C-terminal Venus tag under UASt control. Hatching-Box: Automated in situ monitoring of Drosophila melanogaster development in standard rearing vials Julian Bigge, Maite Ogueta, Luis Garcia, Benjamin Risse Large dimension, high-resolution imaging is important for neural circuit visualisation as neurons have both long- and short-range patterns: from axons and dendrites to the numerous synapses at Oct 11, 2011 · We thank Robert Ray, Mike O'Connor, Julian Ng, Dominique Ferrandon, the Vienna Drosophila RNAi Center, and the Bloomington Drosophila Stock Center for providing flies. 4), is present on the same chromosome11,12. Dec 19, 2016 · Recently we developed an alternative approach using genetically encoded chemical tags CLIP, SNAP, Halo and TMP for tissue labeling; this resulted in >100 fold increase in labeling speed in both mice and Drosophila, at the expense of a considerable drop in absolute sensitivity when compared to optimized immunofluorescence staining. Johnston , Ben Sutcliffe , Julian Ng , Gregory S. qmlb9kfr8sr62hx4vmiyt9vt4rcnfp1mrhskxcxbwlqbob